Fig. 6: STM3 directly activates the expression of FUL1.
From: SISTER OF TM3 activates FRUITFULL1 to regulate inflorescence branching in tomato
a Relative expression of FUL1 in reproductive meristematic tissues of NIL-MIB1CC, NIL-mib1MM, and two STM3OE lines. b Schematic diagram of the promoters of FUL1. Red triangles indicate the SOC1-binding motif. Blue lines indicate primers used for ChIP-qPCR assay, and short red lines indicate DNA probes used for EMSA. The translational start site (ATG) is shown at position +1. c ChIP-qPCR assays showing the enrichment of STM3 at the promoter of FUL1. The immunoprecipitated chromatin was analyzed by qPCR using gene-specific primers as indicated in (b), and IgG was used as the negative control. The intergenic region around ACTIN was used as an internal control. Error bars represent the SD of three biological replicates. Data were compared by two-tailed Student’s t-test, **P < 0.01, error bar, SD. d EMSA showing that STM3 interacts with the probe containing the CArG box on the FUL1 promoter. e Dual-luciferase reporter assay showing that STM3 activates FUL1 expression in tobacco leaves. Fold changes of relative luciferase activity are shown; mu, mutated. Data were compared by two-tailed paired Student’s t-test, **P < 0.01, error bar, SEM.
