Fig. 2: Morphological characterization of PvWOX3a-overexpressing transgenic plants.
From: Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height
a The expression levels of PvWOX3a in transgenic lines revealed by qRT–PCR. Switchgrass PvUbq2 was used for normalization. Values are means ± SEs (n = 3). b Gross phenotypic characterization of switchgrass plants overexpressing PvWOX3a (WOX3aOE). Control plants carried the pANIC6B empty vector. Scale bar = 5 cm. Leaf 3 at the E4 stage (c) and Internode 3 at the E4 stage (d) of control and WOX3aOE transgenic plants are shown. Scale bar = 5 cm. Three-month-old tillers were used to measure plant height (e), diameter of Internode 3 (f), length of Leaf 3 (g), and width of Leaf 3 (h). Three tillers from the same plant were measured for each replicate. Fresh weight biomass yield (i) and dry-weight biomass yield (j) of transgenic switchgrass plants. The control plants and WOX3aOE transgenic plants were harvested after 4 months of growth in the greenhouse. Values are means ± SEs (n = 6). Asterisks represent significant differences determined by Student’s t test. ****p < 0.0001; ***p < 0.0002
