Fig. 4: Overexpression of PvWOX3a downregulated GA2ox and promoted cell elongation in switchgrass.
From: Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height
a Longitudinal section of Internode 2 at the E3 stage of control and WOX3aOE transgenic plants. Scale bar = 100 μm. b Cell lengths based on longitudinal sections of E3I2 of control and WOX3aOE transgenic plants. Values are means ± SEs (n = 18). c The expression levels of PvGA2ox3 and PvGA2ox7 in three WOX3aOE lines were revealed by qRT–PCR. Switchgrass PvUbq2 was used for normalization. Values are means ± SEs (n = 3). d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls. e Dual-luciferase assay showing the repression of PvGA2ox3 and PvGA2ox7 by the PvWOX3a effector construct compared to the control effector construct. Values are means ± SEs (n = 3). qRT–PCR analysis of PvWOX3a (f), PvGA2ox3 (g), and PvGA2ox7 (h) expression levels in wild-type switchgrass plants treated with 200 μm GA3. Switchgrass PvUbq2 was used for normalization. Values are means ± SEs (n = 3). i qRT–PCR analysis of endogenous PvWOX3a expression levels in control plants and WOX3aOE lines. Values are means ± SEs (n = 3). The asterisks represent significant differences in b and e, as determined by Student’s t test. ****p < 0.0001; **p < 0.0021. Asterisks represent significant differences in c, f, g, and h as determined by one-way ANOVA. *p < 0.0332; ***p < 0.0002; ****p < 0.0001
