Fig. 5: PbrMPK18 interacts with PbrCrRLK1L13. | Horticulture Research

Fig. 5: PbrMPK18 interacts with PbrCrRLK1L13.

From: PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes

Fig. 5

A PbrMPK18 interacts with the intracellular domain of PbrCrRLK1L13 in yeast two-hybrid assays. The intracellular domain of PbrCrRLK1L13 fused to the pGADT7 vector and that of PbrMPK18 fused to the pGBKT7 vector. Growth in the medium that lacked Trp, Leu, His, and Ade indicated protein–protein interactions. B PbrMPK18 and PbrCrRLK1L13 interact in the plasma membrane of N. benthamiana leaf epidermal cells in the LCI assay. Fluorescence on the leaf surface indicated protein–protein interactions. C PbrMPK18 and PbrCrRLK1L13 interact in the plasma membrane of N. benthamiana leaf epidermal cells in the BiFC assay. The GFP signal indicates the interaction between PbrMPK18 and PbrCrRLK1L13. Chl represents chloroplast autofluorescence. Merge represents the merged image of GFP, Chl, and bright field. Scale bars = 5 μm

Back to article page