Fig. 2: Modulation of microtubules alters harpin-induced microtubule degradation in V. rupestris cells expressing the GFP-AtTUB6 marker.

Geometric projections from z-stacks collected from representative cells imaged by spinning-disc confocal microscopy are shown after pre-treatment with 10 μM taxol (C), 10 μM oryzalin (E), 0.1% DMSO as solvent control (A), and water as mock control (G) for 30 min, respectively. Then, the cells were treated with 9 μg/ml harpin (B, D, F) for 1 h. Quantitative analysis of microtubule integrity (H). Data represent mean and standard error (SE) of the mean from at least four independent experimental series with 12 to 20 individual cells for each treatment. Significant differences (tested by a Student’s t test) are indicated by *P < 0.05; **P < 0.01; ***P < 0.001