Table 2 Alignment performance of JRGv0 and GRCh38 + decoyJRGv0
From: Construction of JRG (Japanese reference genome) with single-molecule real-time sequencing
(a) The comparision of alighment ratio with GRCh38, GRCh38 + decoyJRGv1 and JRGv1 | ||||
|---|---|---|---|---|
Mean | S.D. | Improvement (compared to total reads) | Improvement (compared to unmapped reads) | |
Alignment ratio with GRCh38 | 96.92% | 0.51% | - | - |
Alignment ratio with GRCh38 + decoyJRGv1 | 97.35% | 0.50% | 0.43% | 16.22% |
Alignment ratio with JRGv1 | 97.36% | 0.50% | 0.44% | 16.47% |
(b) The comparision of alignment reads with GRCh38, GRCh38 + decoyJRGv0 and JRGv0 | |||
|---|---|---|---|
Mean | S.D. | Improvement | |
Alignment reads with GRCh38 | 562,209,444 | 19,701,509 | - |
Alignment reads with GRCh38 + decoyJRGv1 | 564,573,770 | 20,176,172 | 2,364,326 |
Alignment reads with JRGv1 | 564,752,883 | 19,766,532 | 2,543,439 |
(c) The comparison of the relative proper alignment ratio and single alignment ratio with JRGv0-GRCh38 and GRCh38 + decoyJRGv0-GRCh38 | ||
|---|---|---|
Diff | S.D. | |
Relative proper alignment ratio improvement from GRCh38 to JRGv0 | 0.382% | 0.060% |
Relative proper alignment ratio improvement from GRCh38 to GRCh38 + decoyJRGv0 | 0.024% | 0.006% |
Relative single alignment ratio difference from GRCh38 to JRGv0 | 0.016% | 0.006% |
Relative single alignment ratio difference from GRCh38 to GRCh38 + decoyJRGv0 | 0.011% | 0.006% |
