Table 2 Sequences of primers used to amplify genomic deletion junctions (gene reference NG_012232.1(DMD_v001)) and corresponding amplicon regions
From: Breakpoint junction features of seven DMD deletion mutations
Patient ID | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon |
|---|---|---|---|
d1 | CATATGGTTTCTGGCCTTAG | TGCTGTGAACTACAAAGCAC | 1,171,882–1,518,667 |
d2 | GGAACAGTATTCTAGGCAGG | CATCCCTCCCTTCTATGAAC | 1,265,936–1,449,576 |
d3 | CACAAGGGTGTTAAGAACTACC | GATAGTTTCAATAATATGACCATG | 1,329,623–1,453,916 |
d4 | CACCTCTTCTCATCTAATTCC | CGATCACAATCTTCTGTGAAG | 1,366,876–1,552,860 |
d5 | CTATGAACAGGTATAAACCTG | CAGGACCAGCTTCTTGAACG | 1,377,531–1,608,719 |
d6 | GCCTATGGTAAGATTGGTTTC | CCCTTGAGAAATATCTCCAAC | 1,427,297–1,563,083 |
d7 | CTCCTATTTCAGCAAGTATC | GACCCTGGTAGGTACATCATG | 1,548,853–1,584,826 |
