Fig. 3 | Hypertension Research

Fig. 3

From: Dopamine D5 receptor-mediated decreases in mitochondrial reactive oxygen species production are cAMP and autophagy dependent

Fig. 3

Mito-ROS production in human RPT cells. A H2O2 production from mitochondria isolated from human RPT cells, with pyruvate and malate as the substrates. Veh vehicle, Fen fenoldopam (D1R/D5R agonist, 1.0 μM, 12 h), Sch Sch23390 (D1R/D5R antagonist, 1.0 μM, 12 h), AA antimycin A (Qi site inhibitor of mitochondrial ETC Complex III, 0.75 µM, 2 h), Cat catalase (10 µM, 30 min prior to AA treatment). n = 4/group, *P < 0.05 vs Veh. B H2O2 production from mitochondria isolated from DRD1- or DRD5-silenced human RPT cells. The cells were treated with vehicle (Veh), Fen (1.0 µM, 12 h), or Sch (1.0 µM, 12 h). n = 4/group, *P < 0.05 vs Veh, #P < 0.05 vs DRD1-silenced RPT cells. C Mito-ROS staining in DRD1-silenced intact human RPT cells treated with Veh or Fen (1.0 μM, 12 h), as indicated. Bar, 10 μm. The images are from one of three independent experiments

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