Fig. 3 | Hypertension Research

Fig. 3

From: Effect of the traditional Chinese medicine Pinggan-Qianyang decoction on SIRT1–PTEN signaling in vascular aging in spontaneously hypertensive rats

Fig. 3

Effect of PGQYD on vascular aging in SHRs. A, B Thoracic aorta vascular aging was observed by SA-β-Gal staining. C–E The plasma SOD content, NADPH oxidase activity, and anti-superoxide anion content in the aortic tissue of SHRs were determined. F Representative images showing immunofluorescence staining of p53 and quantification of the p53-positive area. G Representative images showing immunofluorescence staining of p21 and quantification of the p21-positive area. H Semiquantitative analysis of p53 and p21 protein expression. J, K. The quantitative expression of p53 and p21 was detected by Western blot analysis. Data are presented as the mean ± SEM (n = 10 per group). Panels show DAPI nuclear staining (blue, middle panels), staining with the corresponding antibody (SIRT1/PTEN) (red, left panels), and merged images (right panels). Original magnification: ×200. DAPI, 4′,6-diamidino-2-phenylindole; EX 527, a specific SIRT1 inhibitor; TCM traditional Chinese medicine

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