Fig. 4 | Hypertension Research

Fig. 4

From: Effect of the traditional Chinese medicine Pinggan-Qianyang decoction on SIRT1–PTEN signaling in vascular aging in spontaneously hypertensive rats

Fig. 4

Immunofluorescence staining of SIRT1 and PTEN in vascular tissue. A Representative image showing immunofluorescence staining of SIRT1 and quantification of the SIRT1-positive area (n = 10). B Representative images showing immunofluorescence staining of PTEN and quantification of the PTEN-positive area (n = 10). Data are presented as the mean ± standard error of the mean. Panels show DAPI nuclear staining (blue, middle panels), staining with the corresponding antibody (SIRT1/PTEN) (red, left panels), and merged images (right panels). Original magnification: ×200. DAPI, 4′,6-diamidino-2-phenylindole; EX 527, a specific SIRT1 inhibitor; TCM, traditional Chinese medicine. C Quantitative analysis of expression of the aging-related protein PTEN, as detected by fluorescence immunohistochemical analysis. D Quantitative analysis of expression of the aging-related protein SIRT1, as detected by fluorescence immunohistochemical analysis.

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