Fig. 1 | Hypertension Research

Fig. 1

From: Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation

Fig. 1

Immunofluorescence and targeted mass spectrometry quantification of renal specimens from patients with malignant nephrosclerosis. A IF microscopy showing the localization of CFD (red), CD34 (green), and DAPI (blue) and merged image showing the co-localization of CFD and CD34 (yellow). B IF microscopy showing the localization of C5b-9 (red), CD34 (green), and DAPI (blue) and merged image showing the co-localization of C5b-9 and CD34 (yellow). C IF microscopy showing the localization and Intensity of CFB, C3 and C4. D Area under the curve (AUC) of RPDSLQHVLLPVLDR, ATLGPAVRPLPWQR, and VQVLLGAHSLSQPEPSK (unique peptides of CFD) showing the relative abundance and spectra showing the co-eluted fragment ions of the corresponding peptides. E AUC of ALPTTYEK, LSPIYNLVPVK, LYYGDDEK, and LPLEYSYGEYR (unique peptides of C5b-9) (C8a, C8b, and C9) showing the relative abundance and spectra showing the co-eluted fragment ions of the corresponding peptides. All data are the mean ± SD; n ≥ 3 per group; *p < 0.05 compared with the expression in the negative control group. Scale bars represent 50 μm

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