Fig. 3 | Hypertension Research

Fig. 3

From: Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation

Fig. 3

Expression and secretion of CFD by CiGEnCs. A, D Protein expression of CFD in CiGEnCs after stimulation with different concentrations of Ang II. B Secretion of CFD in the supernatant of CiGEnCs stimulated with different concentrations of Ang II for 12 h, 24 h, and 36 h. C mRNA expression of CFD in CiGEnCs incubated for 24 h with the negative control (white bars), 0.1 μm Ang II, (gray bars), and 1 μm Ang II (black bars). E Quantitative analysis of western blots for the secretion of CFD from CiGEnCs incubated in serum-free medium, 0.1 μM Ang II, and 1 μM Ang II for 0 h (white bars), 12 h (light gray bars), 24 h (dark gray bars), and 36 h (black bars). All data are the mean ± SD; n ≥ 3 per group; **p < 0.01 and ***p < 0.001 compared with secretion with serum-free medium; #p < 0.05 and ###p < 0.001 compared with incubation with 0.1 μM Ang II

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