Fig. 4 | Hypertension Research

Fig. 4

From: Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation

Fig. 4

Reduction in local complement activation with gene silencing of CFD in CiGEnCs in vitro. A mRNA expression of CFD in CiGEnCs cultured for 24 h. B IF diagram showing the localization of C5b-9 (green), CD34 (red) and DAPI (blue). The merged image showing the colocalization of C5b-9 and CD34 (yellow). C Secretion of CFD in the supernatant of CiGEnCs cultured for 24 h. D Quantitative analysis of western blots for the secretion of CFD from CiGEnCs cultured for 24 h. SFM serum-free medium; All data are the mean ± SD; n ≥ 3 per group; ***p < 0.001 compared with the expression in the mock group. Scale bars represent 20 μm

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