Fig. 5

LOX-1-dependent synergistic effect of AII and high fat diet (HFD) on aldosterone synthesis gene expression, independent of plasma aldosterone levels, in long-term treatment. A Schematic protocol for the animal experiments: Eight-week-old male WT and LOX-1 KO mice were fed either a normal diet (ND) or HFD for 6 weeks. At 10 weeks of age, the mice received a 4-week infusion of either saline or AII at a subpressor dose of 0.1 γ, delivered via subcutaneously implanted osmotic pumps. At the end of the infusion period, the animals were sacrificed, and blood samples and adrenal glands were collected for measurement of plasma aldosterone concentration and real-time qPCR analysis to evaluate the expression of aldosterone synthesis-related genes. B Plasma aldosterone levels in 8-week-old WT and LOX-1 KO mice following the treatment protocol described in Fig. 5A. C Real-time qPCR analysis for gene expression of aldosterone synthesis-related molecules (CYP11B1, CYP11B2, CYP21A1, Hsd3B1, Hsd3B2, AT1a, AT1b, and LOX-1) in the adrenal glands harvested from WT and LOX-1 KO mice after 4 weeks of treatment, as detailed in Fig. 5A. Data are presented as mean ± SEM. Statistical differences were assessed using one-way ANOVA followed by Tukey’s multiple comparison test (B and C) (n = 6–8 per group)