Fig. 5 | Hypertension Research

Fig. 5

From: Goreisan attenuates cardiac hypertrophy and diastolic dysfunction in heart failure with preserved ejection fraction induced by HFD/L-NAME via regulation of ICAT-β-catenin/ERK axis

Fig. 5

GRS suppressed ISO-induced increases in β-catenin and cardiomyocyte hypertrophy. A Representative immunoblots and quantitative analysis of β-catenin and CBB in NRVMs treated with or without GRS (100 μg/mL) in the presence or absence of ISO (10 μM) for 48 hours (n = 11). B Cell surface area of NRVMs treated with or without GRS (100 μg/mL) in the presence or absence of ISO (10 μM) for 48 hours (n = 5). A minimum of 100 cardiomyocytes from each culture dish was measured. Scale bar, 50 µm. C Representative immunoblots and quantitative analysis of ICAT, His-Tag, β-catenin, phosphorylated ERK, ERK, and CBB in NRVMs treated with or without adenovirus harboring ICAT (1 MOI) in the presence or absence of ISO (10 μM) for 48 hours (β-catenin: n = 11, phosphorylated ERK: n = 4). D Cell surface area of NRVMs treated with or without adenovirus harboring ICAT (1 MOI) in the presence or absence of ISO (10 μM) for 48 h (n = 5). A minimum of 100 cardiomyocytes from each culture dish was measured. Scale bar, 50 µm. *p < 0.05, **p < 0.01: post hoc Tukey’s comparison test

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