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A transcription factor coordinating internode elongation and photoperiodic signals in rice

Nature Plants volume 5, pages 358–362 (2019)Cite this article

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Abstract

In several plant species, inflorescence formation is accompanied by stem elongation. Both processes are accelerated in rice upon perception of shortening days. Here, we show that PREMATURE INTERNODE ELONGATION 1 (PINE1), encoding a rice zinc-finger transcription factor, reduces the sensitivity of the stem to gibberellin (GA). The florigens reduce PINE1 expression to increase stem responsiveness to GA and promote flowering. These data indicate the existence of a regulatory network coordinating flowering and GA-dependent growth.

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Fig. 1: Identification of PINE1 and regulation of its expression by photoperiodic and florigenic signals.
Fig. 2: Mutation of PINE1 increases the sensitivity of the stem to GAs and induces stem elongation.

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Data availability

The RNA-seq data that support the findings of this study have been deposited in the GEO with the series record number GSE90493.

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Acknowledgements

We are grateful to S. Tamaki for technical assistance with some experiments. The pACTIN vector was kindly provided by L. Dreni (IBMCP, CSIC, University of Valencia, Valencia) and the CRISPR–Cas9 vector by L.-J. Qu (Peking University and National Plant Gene Research Center, Beijing). We thank A. Costa and the NOLIMITS microscopy facility for technical support. This work was supported by an ERC Starting Grant (no. 260963) to F.F.

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Authors and Affiliations

  1. Department of Biosciences, University of Milan, Milan, Italy

    Jorge Gómez-Ariza, Vittoria Brambilla, Giulio Vicentini, Martina Landini, Martina Cerise, Roshi Shrestha, Francesca Galbiati, Elisabetta Caporali & Fabio Fornara

  2. Department of Agricultural and Environmental Sciences—Production, Territory, Agroenergy, University of Milan, Milan, Italy

    Vittoria Brambilla & Remo Chiozzotto

  3. Instituto de Biologıa Molecular y Celular de Plantas (IBMCP), Universidad Politecnica de Valencia-Consejo Superior de Investigaciones Cientıficas (CSIC), Valencia, Spain

    Esther Carrera & Isabel López Díaz

Authors
  1. Jorge Gómez-Ariza
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  2. Vittoria Brambilla
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Contributions

J.G.-A., V.B., G.V., M.L., M.C., R.S. and F.G. performed the experiments, generated and analysed the transgenic plants and performed the hormone treatments. R.C. analysed the RNA-seq data. E.Carrera and I.L.D. quantified the bioactive GAs. E.Caporali performed the scanning electron microscopy. F.F. and J.G.-A. conceived the project and wrote the manuscript.

Corresponding author

Correspondence to Fabio Fornara.

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The authors declare no competing interests.

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Journal peer review information Nature Plants thanks Junko Kyozuka and other anonymous reviewers for their contribution to the peer review of this work.

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Supplementary information

Supplementary Information

Supplementary Figures 1–10 and Supplementary Table 1.

Reporting Summary

Dataset S1

RNA-seq of Nipponbare and T65 shoot apices during meristem commitment.

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Gómez-Ariza, J., Brambilla, V., Vicentini, G. et al. A transcription factor coordinating internode elongation and photoperiodic signals in rice. Nat. Plants 5, 358–362 (2019). https://doi.org/10.1038/s41477-019-0401-4

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