Extended Data Fig. 1: DCL1, HYL1 and SE promote the precise generation of pre-miRNA 3’ ends.
From: Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis
a, Percentage of Class I pre-miRNAs with intact 3’ ends in different genetic backgrounds (replicate 2 and replicate 3). Each dot in the scatter plot represents one pre-miRNA. The reads whose genome-matched 3’ ends were consistent with the annotated ends with or without non-templated addition were defined as ‘intact’. The black and gray horizontal lines represent the median and the quartiles (first and third quartiles), respectively. Values are Class I pre-miRNAs from one biological repeat (n = 24). Differences between WT (Col-0) and mutants were evaluated by Wilcox. test, two-sided, *P < 0.05; **P < 0.01. See Fig. 1 for biological replicate 1. b, The percentage of individual Class I pre-miRNAs with intact 3’ ends as determined by 3’ RACE-seq in different genetic backgrounds. The reads whose genome-matched 3’ ends were consistent with the annotated ends with or without non-templated addition were defined as ‘intact’. Error bars are s.e.m., n = 3. Differences between WT (Col-0) and mutants were evaluated by student t-tests (paired, two-sided). *P < 0.05; **P < 0.01.
