Extended Data Fig. 1: Cryo-EM data processing of CRY2 tetramer.
From: Structural insights into the photoactivation of Arabidopsis CRY2
Processing of 3036 movie stacks resulted in a total number of 3,252,248 particles. After 2D classification, 3,178,552 particles were kept and subjected to 3D classifications. The best 209,604 particles from one class were retained for further 3D auto-refinement for which C2 symmetry was applied, resulting in a 3.1 Å density map estimated on the basis of the gold-standard Fourier shell correlation with 0.143 criterion.
