Extended Data Fig. 2: Functional analyses of OsTPS28, OsCYP71Z2 and OsCYP71Z21.
From: Selection of a subspecies-specific diterpene gene cluster implicated in rice disease resistance
a, Gas chromatography of the reaction products of OsTPS28 with GGDP. GGDP, geranylgeranyl diphosphate. Casbene and neocembrene reference compounds were purified from infiltrated N. benthamiana leaves by the method described previously26. Compound 1, casbene; Compound 2, neocembrene. b, Gas chromatography of in vitro enzyme assays showing the 10-keto-casbene C5 oxidase activity of yeast-expressed CYP71Z2 in the present of NADPH. Microsomes prepared from yeast containing PESC-URA empty vector were used as a negative control. 10-keto-casbene reference compound was purified from rice leaves by the method described previously13,14. Compound 3, 10-keto- casbene; Compound 4, 5,10-diketo-casbene. c, Gas chromatography of the extracts prepared from the leaves of N. benthamiana infiltrated with OsTPS28 overexpressing vector.
