Extended Data Fig. 5: The accumulation of HSO4-JA is determined by the expression of ST2a.
From: A light-dependent molecular link between competition cues and defence responses in plants
FR fails to upregulate the pool of HSO4-JA in a pif4 pif5 pif7 triple knock-out mutant, where FR fails to upregulate ST2a expression. Samples for metabolite analysis were taken 4 h after wounding. The dotted/dashed lines parallel to the abscissa indicate the basal levels of HSO4-JA in plant of both genotypes harvested before the wounding treatment. Bars indicate means for wounded plants; thin bars = 1 SE; n = 6 independent pools of 3 rosettes each (small open circles). The interactive effect of genotype and light were tested using two-way ANOVA; different letters indicate significant differences between means. b, In Col-0 plants, there is a tight, significant positive correlation between ST2a mRNA and HSO4-JA concentration in rosette tissues (two-tailed Spearman test). Different levels of ST2a gene expression were obtained by varying the amount of mechanical damage (see Methods for details). Samples were taken 4 h after wounding (each datum point represents an independent pool of 3 rosettes).
