Extended Data Fig. 1: Comparative analyses of rhythmic circadian oscillation in shoots and roots.
From: A mobile ELF4 delivers circadian temperature information from shoots to roots
Luminescence of a, LHY::LUC (n=6 for Sh, n=6 for Rt), and b, TOC1::LUC (n=16 for Sh, n=15 for Rt) rhythms simultaneously measured in shoots (Sh) and roots (Rt). Root luminescence signals in a, are represented in the right Y-axis. c, Circadian period (left Y-axis, n=16 for Sh, n=14 for Rt) and amplitude (right Y-axis, n=16 for Sh, n=15 for Rt) estimates of TOC1::LUC luminescence signals (data are represented as the median ± max and min; 25-75 percentile). *** p-value<0.0001; two-tailed t-tests with 95% of confidence. d, Luminescence of CCA1::LUC rhythms in elf4-1 Rt (n=8) (from Fig. 1d) showing the weak rhythms of the mutant. e, Circadian time course analyses of ELF4 mRNA expression in WT and elf4-1 mutant Rt. f, Luminescence of LHY::LUC rhythms in WT (n=6) and elf4-1 mutant Rt (n=6). g, Circadian time course analyses of LHY mRNA expression in roots of WT and elf4-1. h, Circadian time course analyses of ELF4 mRNA expression in WT and elf4-1 mutant Sh (also in Extended Data Fig. 3c). i, Luminescence of LHY::LUC (n=6) and j, CCA1::LUC (n=9) rhythms in WT and elf4-1 mutant Sh. k, Circadian period estimates of LHY::LUC in WT (n=12) and ELF4-ox (n=14) roots; data are represented as the median ± max and min; 25-75 percentile). *** p-value<0.0001; two-tailed t-tests with 95% of confidence. a-b, d-j, Data are represented as the means + SEM. The mRNA expression and promoter activity analyses were performed under constant light conditions previous synchronization of plants under LD cycles at 22°C. The “n” values refer to independent samples. a-k, Two biological replicates were performed for all experiments, with measurements taken from distinct samples grown and processed at different times.
