Extended Data Fig. 2: RNA-Seq analyses of WT and elf4-1 mutant roots.
From: A mobile ELF4 delivers circadian temperature information from shoots to roots
a, Quantitative analysis of DEGs in elf4-1 versus WT roots. The statistical analyses of the DEGs are detailed in Materials and Methods. b, Heatmap of the median-normalized expression (Z-scaled FPKM values) of the oscillator genes in WT and elf4-1 roots. Analyses by RT-QPCR of c, ELF4, d, PRR7, e, CCA1 and f, LUX mRNA expression in roots at CT75 after three days in LL. Visualization of RNA-seq reads by using the Integrative Genomics Viewer (IGV) browser for g, ELF4, h, PRR7, i, and CCA1. j, Number of lateral roots per cm in the Ws-2 (WT, n=86), elf4-1 (n=28) or ELF4-ox lines (n=48). Plants were grown for 12 days under short day (8h light:/16h darkness) photoperiod with a constant temperature of 22°C. Letters signify significant difference (p<0.01) as determined by a one-way ANOVA with a Tukey HSD post hoc test. Data is represented as the median ± max and min; 25-75 percentile). Data represents an average of two independent experiments.
