Extended Data Fig. 10: Overexpression of Venus-D6PKS345D rescued the defects of lateral root formation and hypocotyl gravitropism in pdk1.1 pdk1.2.
From: The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis
a, b, 35S::Venus-D6PKS345D (L3 as a representative line) rescued the lateral root defects of pdk1.1 pdk1.2. Nine-day-old seedlings were observed. n = 3 biologically independent experiments, with similar results obtained. A representative photo is shown in (a, scale bar, 2 cm), and the lateral root number was quantified (b). Dots represent individual plants, and lines indicate mean ± s.d.. n = 15, 15, 14 and 14 individual seedlings for Col-0, pdk1.1 pdk1.2, 35S::Venus-D6PKS345D in pdk1.1 pdk1.2 (L3), and 35S::Venus-D6PKS345D in pdk1.1 pdk1.2 (L5), respectively. Different letters represent significant difference, P < 0.05, by one-way ANOVA with a Tukey multiple comparison test, and P values are shown for each genotype compared with Col-0. c, The subcellular localisations of Venus-D6PK, Venus-D6PKS345A, and Venus-D6PKS345D exhibit difference in PM targeting, but not show difference in the basal polarity. Four-day-old seedlings of p35S::Venus-D6PK, p35S::Venus-D6PKS345A, and p35S::Venus-D6PKS345D, in Col-0 and pdk1.1 pdk1.2 respectively, were imaged by CLSM. The “Green Fire Blue” LUT was used for photo visualization based on fluorescence intensity by Fiji. Representative photos of at least three independent transgenic lines were shown. Scale bars, 10 µm. n = 2 biologically independent experiments, with similar results obtained.
