Extended Data Fig. 6: Biological significance of the MpARF LFG motifs.

a, Ten-day-old gemmalings of proMpARF1:ARF11Δ1 (11Δ1), proMpARF1:ARF12Δ1 (12Δ1) and proMpARF1:ARF13Δ1 (13Δ1) lines. The experiment was repeated twice with similar results. b, Relative qPCR expression of WIP gene in Tak-1 wild-type, proMpARF1:ARF12Δ1 (12Δ1) and proMpARF1:ARF13Δ1 (13Δ1) gemmalings, 0, 1, 2 and 4 hours after treatment with 10 µM 2,4-D. The experiment was performed once with three technical replicates. c, Relative qPCR expression of YUC2 gene in Tak-1 wild-type, Mparf1-4 and proMpARF1:ARF11Δ1 (11Δ1) gemmalings, after a 1-hour treatment with control media or with 10 µM 2,4-D. n = 3 technical replicates. The experiment was repeated twice with similar results. Bar in (a) = 2 mm.