Extended Data Fig. 5: RBR functions cell-autonomously in the RM. | Nature Plants

Extended Data Fig. 5: RBR functions cell-autonomously in the RM.

From: An inducible genome editing system for plants

Extended Data Fig. 5

a, A three-day mock treatment of ip35S»Cas9p-RBR in RBR-YFP; amiGORBR. b, A one-day induction caused a reduced RBR-YFP signal mainly in the root cap region without an obvious phenotype. c, A three-day induction of RBR editing with ip35S typically led to LRC overproliferation (white arrows) without affecting the YFP signal in other domains. While half of the transformants showed sectors of variable size lacking RBR-YFP expression (left panel in c), the other half showed almost complete absence of RBR-YFP in the domain of ip35S (right panel). Cell walls are visualized by calcofluor. Numbers indicate the frequency of the observed phenotype in independent T1 samples. Experiments were repeated three times. Scale bar, 50 μm.

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