Extended Data Fig. 7: GNOM is required for PIN1 polarity and expression. | Nature Plants

Extended Data Fig. 7: GNOM is required for PIN1 polarity and expression.

From: An inducible genome editing system for plants

Extended Data Fig. 7

a, GNOM expression disappeared from the vasculature after a 6-day induction of ipWOL»Cas9p-tagRFP-GNOM in GN-GFP. Due to the weak GFP signal, only roots showing a clear loss of GFP signal were included in quantification. b, A three-day induction of ipWOL»Cas9p-tagRFP-GNOM in PIN1-GFP resulted in loss of polarity and decreased expression of PIN1-GFP in the endodermis (en), pericycle (p) and stele (s) (white arrows). Right panels are magnified images of the regions marked with a red box in the left panels. Cell walls are marked by calcofluor. Numbers indicate the frequency of the observed phenotype in independent T1 samples analyzed. Experiments were repeated three times. Scale bar in right panels of a, 25 μm; others, 50 μm.

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