Extended Data Fig. 4: myo-Inositol preferentially influences Bacillus megaterium (Related to Fig. 4).
a, Root colonization of the bacteria including B. megaterium YC4-R4, B. megaterium TG1-E1, B. amyloliquefaciens GB03 and P. syringe DC3000. The effects of myo-inositol on bacteria colonization to rdd roots were statistically analyzed based on each bacteria strain. The bar graph shows representative data from three independent experiments (n = 3 biological independent samples). Sets of data were analyzed by one-way ANOVA including a Tukey’s test, where * means statistical difference by p < 0.05. b, Gene Ontology (GO) categorization of DEGs that were up-regulated in myo-inositol-treated YC4-R4. c, GO categorization of DEGs that were down-regulated in myo-inositol-treated YC4-R4. d, Relative expression levels of a group of key genes involved in bacteria colonization-related activities. The bar graphs show representative quantitative real-time PCR results from three independent experiments (n = 3 biological independent samples). Sets of data were compared by t-student test by two-tailed way and 95% confident intervals (p < 0.05), in which * means statistical differences with mock set. In all data sets described above, error bar means SD.
