Extended Data Fig. 1: DNA strand break-inducing reagents attenuate the growth of protonemata.
From: DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella
a A representative wild-type leaf at 0, 12, 24, 36, 48, 60, and 72 hours after excision. Blue asterisks indicate the positions of chloronema apical stem cells generated from the edge cells of the excised leaf. The reprogramming of excised leaves was observed in more than two independent experiments with similar results. b–d Representative one-week-old wild-type plants on solid BCDAT medium with or without various concentrations of zeocin (b), bleomycin (BLM) (c), or camptothecin (CPT) (d). Protonema growth slowed under the treatment with 0.5 µg/mL zeocin, 0.1 µg/mL BLM, or 200 nM CPT. Three plants in each concentration of zeocin, BLM, or CPT were observed twice independently with similar results. Scale bars: 100 µm in (a), 1 mm in (b–d).
