Extended Data Fig. 5: Induction of CYCD1;1, CSP1, STEMIN1, and closely related genes in reprogramming cells after transient zeocin treatment.
From: DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella
a Representative gametophore leaves of CYCD1;1pro:NGG (NLS-sGFP-GUS) #26313, nPpCSP1-Citrine-3ʹUTR #115, STEMIN1pro:NGG #7, STEMIN2pro:NGG #238, and STEMIN3pro:NGG #262 after 6 hours of 50 µg/ml zeocin treatment. Bright-field images taken at 10 and 72 h and fluorescent images taken at 10, 24, or 25, 44, 64, and 72 h from time-lapse images (Supplementary Videos 3-7) are shown. White arrows in the middle panels indicate chloronema cells near the observed leaf. The experiments were performed twice independently with similar results. Scale bars: 200 µm. b Intensities of sGFP or Citrine signals in three independent protruded and un-protruded cells of intact gametophore leaves of the lines described in (a) after zeocin treatment. Signal intensity was measured with Fiji 1.0. Red arrows indicate time points at which the cells began to protrude.
