Extended Data Fig. 3: Sequence alignment of the GAF domain of PHYC in foxtail millet and its homologs. | Nature Plants

Extended Data Fig. 3: Sequence alignment of the GAF domain of PHYC in foxtail millet and its homologs.

From: A mini foxtail millet with an Arabidopsis-like life cycle as a C4 model system

Extended Data Fig. 3: Sequence alignment of the GAF domain of PHYC in foxtail millet and its homologs.

Alignment was carried out using Clustal W method of the MegAlign software. Red box indicates the conserved residue Leu across all listed species that is substituted with His in xiaomi-2, demonstrating its functional importance for PHYC. Accession numbers for the aligned sequences: Arabidopsis thaliana NP_198433, Brachypodium distachyon XP_003559446, Brassica napus XP_013680236, Ipomoea nil XP_019162785, Oryza sativa AAF66603, Panicum miliaceum, RLN42126, Solanum lycopersicum NP_001307446, Sorghum bicolor XP_002466441, Triticum aestivum AAU06208, Vitis vinifera ACC6096 and Zea mays XP_008665426. PHYC protein in Jingu21 is presented as for Setaria italica (Si9G09200).

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