Extended Data Fig. 7: Pull-down assay of the interaction between TFL1 and the dominant-negative (DN-ran2) or truncated versions of RAN2.
From: Mobile TERMINAL FLOWER1 determines seed size in Arabidopsis
a, Schematic diagrams of DN-ran2 and RAN2 truncated (Del1-Del3) proteins that were fused to MBP. The full-length RAN2 protein contains the effector binding domain and acidic C-terminal domain implicated in protein-protein interaction. The DN-ran2 protein contains a mutation of threonine (T) at the residue 27 to asparagine (N), which is located near the effector-binding domain of RAN2. b, Pull-down assay result. MBP and various MBP fusion proteins were used as baits, and the corresponding loading control was stained with Ponceau S (lower panel). The input of the prey protein TFL1-4HA extracted from 35 S:TFL1-4HA siliques and its corresponding pull-downed signals were examined by immunoblot analysis using anti-HA antibody (upper panel). The experiment was repeated three times independently with similar results.
