Extended Data Fig. 5: Knockout mutation in GORKY has a major impact on the SGA profile of ripe fruit.
From: The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness
Generation of loss-of-function GORKY lines through CRISPR/Cas9 gene editing. a, Position of the four guide RNAs targeting the third GORKY gene exon are indicated. b, Examples of mutations generated by editing are depicted below. PAM sequence is indicated in red; target sequence is depicted in blue and the adjacent nucleotides are shown in black. Dots represent the deletions. c, The levels of α-tomatine and its hydroxy- and acetoxy-SGA derivatives are significantly increased in gorky ripe fruit. Three independent T1 generation transformants were analyzed (#1, #2 and #3). Bars represent mean ± SE (n=3 for WT, n=4 for line #1 and #3, n=7 for line #2); asterisks indicate significant changes compared to WT (non-transformed) fruit samples as calculated by two-tailed unpaired t-test (*P value < 0.05; **P value < 0.01;***P value < 0.001; ****P value < 0.0001). LC-qTOF was employed for SGA analysis.
