Extended Data Fig. 8: Overexpression of GORKY in the bitter accession EA07290 background restores the SGA profile to red, ripe fruit.
From: The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness
The levels of α-tomatine and dehydrotomatine are significantly decreasing in GORKY-Ox (in the EA07290 background fruit). On the other hand, levels of esculeoside A and dehydroesculeoside A, characteristic to WT red, ripe fruit, dramatically increase. SGA levels for plants from two independent tomato transgenic T1 lines are depicted (#1 and #2). In line #1 the levels of hydroxy-and acetoxytomatine are significantly decreased, while the level of acetoxy-hydroxytomatine is increased. In both lines, the levels of di-hydroxytomatine are increased. Bars represent mean ± SE (n=4 for WT, n=3 for lines #1, #2), asterisks indicate significant changes compared to WT (non-transformed EA07290) samples as calculated by unpaired two-tailed t-test (*P value < 0.05; **P value < 0.01;***P value < 0.001; ****P value < 0.0001). LC-qTOF was employed for SGA analysis.
