Extended Data Fig. 1: At4g32950 is an Arabidopsis PP2C subfamily E protein.
From: A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1
a, A combined phylogenetic tree of PP2C subfamily E proteins from Arabidopsis (At), potato (St), Medicago truncatula (Mt), rice (Os), maize (Zm) and moss Physcomitrella patens (Pp). At4g32950 was classified into a distinct clade (shaded) that includes members from potato, Medicago, rice and maize, but not mosses. Numbers represent bootstrap values from 1,000 replicates. b, Expression stability of two different reference genes, EF-1α and TUA4, between WT and cepd1,2 cepdl2 triple mutant plants. TUA4 and EF-1α expression level in WT and cepd1,2 cepdl2 was quantified by RT-qPCR using EF-1α and TUA4 as reference genes, respectively. c, Expression of NRT2.1 in WT and cepd1,2 cepdl2 triple mutant plants. Data are presented as the mean ± SD, and each dot represents a biological replicate. P-values are 2.76 × 10−2 for 3 mM and 1.61 × 10−4 for 1 mM. For (b) and (c), statistical significance is determined by two-tailed non-paired Student’s t test (*P < 0.05, ‘ns’ indicates not significant).
