Extended Data Fig. 2: Phenotypic analysis of ceph-1 mutant plants.
From: A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1
a, Fresh weight of 14-day-old shoots of WT, ceph-1 mutant, and ceph-1 plants complemented with CEPH grown on 1 mM NO3– medium. b, Fresh weight of 14-day-old shoots of WT, ceph-1 mutant, and ceph-1 plants complemented with CEPH-GFP grown on 1 mM NO3– medium. c, Root phenotypes of 10-day-old WT and ceph-1 mutant plants grown on 1 mM NO3– medium. Scale bar = 1 cm. d, Primary root length of 10- or 14-day-old WT and ceph-1 mutant plants grown on 1 mM NO3– medium. e, Ratio of total lateral root (LR) length to primary root (PR) length of 10- or 14-day-old WT and ceph-1 mutant plants grown on 1 mM NO3– medium. P-values are 2.23 × 10−2 for 10 d and 4.01 × 10−3 for 14 d. f, Phenotypes of 14-day-old WT and ceph-1 mutant plants grown under N-replete conditions (10 mM NH4+, 10 mM NO3–). Scale bar = 5 mm. g, Fresh weight of the shoots and roots described in (f). (h) HATS and calculated LATS NO3– uptake activities of 14-day-old WT and ceph-1 mutant plants grown on 1 mM NO3– medium. P-values are 6.13 × 10−5 for HATS and 1.32 × 10−3 for LATS. i, Expression of nitrate transporter genes in WT and ceph-1 mutant plants. j, Expression of CEPD1/2 and CEPDL2 in WT and ceph-1 mutant plants. P-value is 3.81 × 10−3 for CEPDL2. k, Induction level of CEPH in XVE-CEPH plants 6 h after estradiol treatment. P-value is 3.65 × 10−3. Data are presented as the mean ± SD, and each dot represents a biological replicate. For (a) and (b), different letters indicate statistically significant differences (P < 0.05, one-way ANOVA with post-hoc Tukey’s test). For (d), (e) and (g)-(k), statistical significance is determined by two-tailed non-paired Student’s t test (*P < 0.05, ‘ns’ indicates not significant).
