Extended Data Fig. 2: HPLC elution profiles of pigment extracts from coupled PSI-cyclase assays.
From: How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls
A complete assay contained 2 µM AcsF, 0.04 mg ml−1 spinach Fd, 14 µM MgPME, spinach PSI containing 6 (1× PSI) or 22.4 µM (~4× PSI) Chl a, 20 µM spinach Pc, 2 mM Asc, 60 µM DCPIP and 0.29 mg ml−1 catalase. Assays were incubated either in the dark for 30 min, or under red light illumination for 15 or 30 min. Pigment extracts from the assays were analysed by HPLC and pigment elution was monitored by fluorescence at 640 nm excited at 440 nm. Pigment species were identified by retention times and fluorescence spectra (as in Fig. 4). See Supplementary Fig. 4a for HPLC analysis of pigment extracts from additional control assays.
