Extended Data Fig. 4: The DWF4 expression maximum precedes the BR signalling peak monitored by the nuclear fluorescence intensity of BES1–GFP.

a, Six-day-old Arabidopsis roots expressing DWF4–GFP/dwf4 and BES1–GFP/Col-0 under the control of their native promoters. Scale bars, 100 µm. b, Fluorescence intensity of DWF4–GFP and BES1–GFP in single cells within single cell files, along the longitudinal root axis shown in a. Note that the DWF4–GFP expression peak precedes the BES1–GFP nuclear accumulation peak in the elongation zone. The DWF4–GFP fluorescence intensity was measured for the whole cells along the trichoblast file whereas the BES1–GFP florescence intensity was measured in the nuclei along cortical file.