Extended Data Fig. 3: Consequence of the additional AT dinucleotide on the predicted VC1 protein.
From: VC1 catalyses a key step in the biosynthesis of vicine in faba bean
An alignment of Hedin/2 VC1 and Mélodie/2 vc1 amino acid sequences is shown. Predicted domains are shown underneath the alignment (RibB, 3,4-dihydroxy-2-butanone-4-phosphate synthase domain; RibA, GTP cyclohydrolase II domain). A measurement of residue conservation is shown underneath the predicted domains, distinguishing between identical residues and other scenarios (non-identical ones as well as gaps/insertions). The position of the AT insertion, which results in a frame shift, is marked with a black triangle underneath the conservation score (position 360). The following key residues in VC1 enzymatic domains are marked: blue arrows, substrate binding in RibB; red arrows, catalysis in RibB; green arrows, Zn binding in RibA; grey arrows, catalysis in RibA; orange arrows, GTP specificity. The prediction of key resides is based on Hiltunen et al.17 and Ren et al.41.
