Fig. 1: ptpTALECD for three plastid genes.
From: Targeted base editing in the plastid genome of Arabidopsis thaliana
a, A pair of pTALECD proteins and its targeting window (shown in a red rectangle) in 16S rRNA gene and CD half combination. Substitution of the fifth and/or eighth C/G pairs (shown in red) with T/A pairs was predicted to confer Spm resistance. b, T-DNA region of the tandem expression vectors for ptpTALECD. c–e, Base-edited plant numbers, editing efficiencies shown in colour (bottom) and predicted amino acid substitutions in the three target windows of 23 DAS T1 plants (c, 16S rRNA; d, rpoC1; e, psbA). f–h, Representative data from Sanger sequencing in the ptpTALECD targeting windows of 23 DAS T1 plants (f, 16S rRNA; g, rpoC1; h, psbA). i, Transition of substitution frequency states at the targeted bases between 11 DAS and 23 DAS T1 plants. Abbreviations: h/c, heteroplasmically or chimaerically substituted; homo, homoplasmically substituted; Cp, preferential target cytosines; and C*, special target cytosines predicted to cause biological effects.
