Extended Data Fig. 9: Effects of Hvmads1 mutation on the expression of key regulators in response to temperature.
From: MADS1 maintains barley spike morphology at high ambient temperatures
a, Heat map showing DEGs relevant to spike development (left) and temperature response (right). b, qRT-PCR analysis of selected genes related to inflorescence meristem identity in W3.5 WT (GP) and hvm1 spikes at 15 °C and 25 °C. OSH1, ORYZA SATIVA HOMEOBOX1; AP1, APETALA 1; VRN, VERNALIZATION; TFL1, TERMINAL FLOWER 1; TB1, TEOSINTE BRANCHED 1; TAW1, TAWAWA1; RPK4, RECEPTOR-LIKE PROTEIN KINASE 4; IDS1, INDETERMINATE SPIKELET 1. c, qRT-PCR analysis of selected genes known to regulate barley spike development, spikelet identity and row-type in W3.5 WT and hvm1 spikes at 15 °C and 25 °C. VRS, SIX-ROWED SPIKE; COM2, COMPOSITUM 2. d, qRT-PCR analysis of selected genes related to temperature response in W3.5 WT and hvm1 spikes at 15 °C and 25 °C. PIF4, PHYTOCHROME-INTERACTING FACTOR 4; ER, ERECTA; TT1, THERMO-TOLERANCE 1. HvActin7 and HvEF2 were used for normalisation. For (b–d), data shown as mean ± s.d., n = 3 biological replicates. P values indicate results from indicated pairwise comparisons of one-way ANOVA tests (b–d).
