Extended Data Fig. 7: SHR acts downstream of TMO5/LHW.
a,b, Confocal images of root meristems expressing pTCSn::ntdTomato reporter in Col-0 and shr-2 backgrounds. c, Quantification of the pTCSn::ntdTomato mean pixel intensity in the mentioned genotypes. Boxes represent the 1st and 3rd quartile and the center line represents the median. n indicate independent plants. Lower-case letters on top of the boxplots indicate significantly different groups as determined by one-way ANOVA with post-hoc Tukey HSD testing (p<0.001). d-g, Expression of pSHR::SHR:GFP in the dGR root meristem grown on mock medium and transferred to mock or 10 μM DEX for 24 h. h, Relative expression levels of SHR in wild type (Col-0), TMO5/LHW misexpression, and tmo5, tmo5 tmo5like1 (t5 t5l1) and tmo5 tmo5like1 tmo5like3 (t5 t5l1 t5l3) mutant backgrounds. Asterisks indicate significance values as determined by standard two-sided t-tests. Boxes represent the 1st and 3rd quartile and the center line represents the median. n = 3 biological replicates. Black lines indicates mean values and grey boxes indicate data ranges. i-l, Microscopic images of xylem differentiation in the mentioned genotypes. p: protoxylem, m: metaxylem. m, Quantification of the different classes of xylem phenotypes shown in panels i-l. n-o, Expression of pSCR::H2B:nYFP in the dGR root meristem grown on mock medium and transferred to mock or 10 μM DEX for 24h. p-q, Expression of pmiR165a::nYFP/GUS in the dGR root meristem grown on mock medium and transferred to mock or 10 μM DEX for 24h. r-s, Relative expression levels of SCR and miR165a in wild type (Col-0), TMO5/LHW misexpression, and tmo5, tmo5 tmo5like1 (t5 t5l1) and tmo5 tmo5like1 tmo5like3 (t5 t5l1 t5l3) mutant backgrounds. Boxes represent the 1st and 3rd quartile and the center line represents the median. n = 3 biological replicates. Asterisks indicate significance values as determined by standard two-sided t-tests. Black lines indicates mean values and grey boxes indicate data ranges. In panels a-b, d-g, i-l and n-q, scale bars are 50 μm. In all panels, n represents the number of replicates or data points; all data and statistics are summarized in Supplementary Data 4. The experiments in d-g, n-q were repeated three times independently, with similar results.
