Extended Data Fig. 2: Characterization of BGLU44 expression and function.
a-c, Predicted expression of LOG4 and BGLU44 according to a single cell atlas of the Arabidopsis root meristem. Note the predicted co-expression in the feature plots for both genes. d-g, Expression of pBGLU44::nYFP/GUS in the dGR shoot and root meristems grown on mock medium and transferred to mock or 10 μM DEX for 24h. h, Inflorescence stem and silique phenotypes in the indicated genotypes. i, Relative expression levels as determined by RT-qPCR of BGLU44 and LOG4 in the indicated lines. Boxes represent the 1st and 3rd quartile and the center line represents the median. Asterisks in graphs indicate significance values as determined by standard two-sided t-tests. Black lines indicates mean values and grey boxes indicate data ranges. j, Expression of pRPS5A::BGLU44:YFP in the root meristem grown on mock medium showing that the BGLU44:YFP fusion protein is expressed. k-o, Phenotypes and quantification of the number of vascular cell files in the mentioned genotypes. Boxes represent the 1st and 3rd quartile and the center line represents the median. Statistics in n are performed using pairwise comparisons using a standard two sided t-test (p-value<0,05). p-s, Confocal images of optical cross sections of root meristems expressing pTCSn::ntdTomato reporter in the mentioned genotypes. Scale bars are 10 μm. t-w, Root hair phenotypes of the mentioned genotypes. In all panels, n represents the number of replicates or data points; all data and statistics are summarized in Supplementary Data 4. The experiments in j was repeated three times independently, with similar results.
