Extended Data Fig. 5: Alignment of the amino acid sequences of ClpP/R subunits.

Sequence alignment of ClpP and ClpR subunits from Escherichia coli (Ec), Synechocystis sp. PCC6803 (Sy), Mycobacterium tuberculosis (Mb), Chlamydomonas reinhardtii (Cr) and Arabidopsis thaliana (At), extracted form an alignment of 243 algal, plant and bacterial sequences. Structural elements derived from the crystal structure of E. coli ClpP are shown on top of the alignment (α: α-helices, β: β-strands). The three catalytic residues are marked by red arrows. The best-conserved residues are shown with a coloured background. Chlamydomonas subunits (names in red) are shown with the experimentally-determined mature N terminus boxed and sections not assigned in the Clp-S1 structure in faded colours. ClpP1_Cr is shown after removal of the insertion sequence IS1 (at pos 59, purple arrow), so the N terminus of ClpP1c, starting near the end of IS1, is not shown. The less conserved regions of Clp subunits used for subunit assignment in Fig. S6B are underlined with red lines. The proline motif region is underlined with a blue line.