Extended Data Fig. 4: Transcriptomic analysis of DEGs between the two NILs.
From: TT2 controls rice thermotolerance through SCT1-dependent alteration of wax biosynthesis
a, Venn diagram and volcano plot of DEGs between the NILs under different treatments. Criteria for defining differentially expressed genes (DEGs) are |log2(Fold Change)| > 0 and P value < 0.05, n = 3 samples. H, heat treatment; C, control treatment. b, Genes clustered by expression level Cluster analysis for the two NILs under normal conditions or heat treatment. FKPM value was adjusted by log2(fpkm+1). The adjusted FKPM of every gene in a subcluster is shown as a gray line. A blue line represents the mean adjusted FKPM of the subcluster. c, Results of KEGG enrichment analysis of subcluster 2 and subcluster 3 are shown. Padj, adjusted P value; n = 3 samples. d, Heat map showing the expression patterns of wax-related DEGs in the two NILs under heat treatment. P < 0.05, n = 3 samples. Each sample represents 24 individual plants (a-d). P value was obtained by wald test using a model based on the negative binomial distribution (a,d), P value in KEGG obtained based on hypergeometric distribution model was adjusted to Padj using the Benjamini & Hochberg method (c).
