Extended Data Fig. 10: Calculation of KD value between SCT1 and CaM.
From: TT2 controls rice thermotolerance through SCT1-dependent alteration of wax biosynthesis
Three concentrations of CaM were used to calculate KD value between SCT1 and CaM fitted to one-to-one evaluation type at the presence of CaCl2 or EGTA. Three lines are fitted to one curve and the KD value was calculated. The signal after blocking the SCT1-CaMBD-immobilized chip was set to 0.
