Extended Data Fig. 2: Molecular identification and phenotypic analysis of TT2 transgenic lines.
From: TT2 controls rice thermotolerance through SCT1-dependent alteration of wax biosynthesis
a, Schematic map of the sgRNA target sites in TT2, and a schematic showing vector construction and sequence alignment of the TT2-knockout transgenic lines with wild-type HJX. b, Relative expression level of TT2 in NIL-TT2HPS32 and pUbi:TT2HJX/NIL-TT2HPS32. P = 5.28 × 10−10 by one-way ANOVA (n = 6). c, Grain length in HJX, TT2KO/HJX, NIL-TT2HPS32, pTT2-gTT2HJX/NIL-TT2HPS32, and pUbi-TT2HJX/NIL-TT2HPS32. Scale bar, 1 cm. d, Survival rate of TT2 transgenic lines. The survival rate was determined after heat treatment (42 °C, RH > 90%) for 24 hr and subsequent recovery at 28 °C for 7 days. For box-and-whisker plots, the central line, box and whiskers indicate the median, IQR and 1.5 times the IQR, respectively. n = 6 samples, 24 individual plants per sample. TT2KO/HJX, P = 1.00 × 10−27; pUbi-TT2HJX/NIL-TT2HPS32, P = 1.09 × 10−24; pTT2-gTT2HJX/NIL-TT2HPS32, P = 2.58 × 10−32 by one-way ANOVA. The same letters indicate no significant difference at P > 0.05 as determined by Tukey’s HSD test (b, d).
