Extended Data Fig. 1: Reduced root growth and root sugar levels in ABA insensitive mutants and sweet11/12 mutant under osmotic stress.
From: Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought
a, Fresh weight of roots from Col-0 wild-type, pyl duodecuple (pyl), snrk2.2/3/6 (snrk2) and sweet11/12 mutants, and SWEETpro::SWEET-HA transgenic plants expressing wild-type SWEET11 or 12 in the sweet11/12 mutant background, without or with mannitol, PEG or ABA treatments. b, Sucrose contents in roots of Col-0 wild-type, pyl duodecuple (pyl), snrk2.2/3/6 (snrk2) and sweet11/12 mutants, and SWEETpro::SWEET-HA transgenic plants expressing wild-type SWEET11 or 12 in the sweet11/12 mutant background, without or with mannitol or ABA treatments. c-d, Root growth of Col-0 wild-type, snrk2.2/3/6 (snrk2) and pyl duodecuple (pyl) mutants, grown on mannitol plates with (right panel) or without (left panel) exogenous sucrose. The primary root length was quantified (d). e-f, Glucose (e) and fructose (f) levels in roots of Col-0 wild-type, pyl duodecuple (pyl), snrk2.2/3/6 (snrk2) and sweet11/12 mutants, without or with mannitol or ABA treatments. g-h, Root growth of Col-0 wild-type and sweet11/12 mutant grown on mannitol plates with (right panel) or without (left panel) exogenous sucrose. The primary root length was quantified (h). Error bars indicate SEM (n = 10 seedlings) in (a, d, h), SEM (n = 5 seedlings) in (b) and SEM (n = 3 seedlings) in (e, f). Student’s t-test (two-sided). ‘a,’ ‘b,’ and ‘c’, significance evaluated by post hoc Tukey test after one way ANOVA (p < 0.05). The experiments in (c and g) were repeated independently at least three times with similar results.
