Extended Data Fig. 2: SnRK2s interact with and phosphorylate SWEET11 and 12.
From: Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought
a, SnRK2s are co-immunoprecipitated with SWEET12 in SWEET12pro::SWEET12-GFP transgenic plants. SWEET12 protein was immunoprecipitated using SWEET12pro::SWEET12-GFP transgenic plants treated without or with 100 μM ABA. b, Coimmunoprecipitation (coIP) assay in Arabidopsis protoplast. Total proteins were extracted from protoplasts co-transfected with HA-SnRK2.6 and FLAG-SWEET11, FLAG-SWEET12 or FLAG empty vector control, and immunoprecipitated using anti-FLAG beads. Coimmunoprecipitated HA-SnRK2.6 was detected with anti-HA antibody. The experiments in (b) were repeated independently at least two times with similar results.
