Extended Data Fig. 3: SnRK2s phosphorylate SWEET11 and 12.
From: Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought
a, Phosphopeptides containing Ser248 from the C-terminus of SWEET12 were detected in SWEET12pro::SWEET12-GFP transgenic plants following ABA treatment. ‘pep score’, peptide score, which determines the probability that the peptide fragments detected by the mass spectrum. b, Phosphorylation of Ser248 of SWEET11 and 12 in Col-0 wild-type and sweet11/12 mutant seedlings, with or without mannitol or ABA treatment. Anti-SWEET11P and anti-SWEET12P indicate anti-phospho-Ser248-SWEET11 and 12 antibodies, respectively. Anti-phospho-Ser248-SWEET11 and 12 antibodies were used to detect phosphorylation of SWEET11 and 12, respectively. Actin was used as a loading control. c,d, Protein abundance of HA-tagged SWEET11 and SWEET12 in SWEET11pro::SWEET11-HA (c) and SWEET12pro::SWEET12-HA (d) transgenic plants. Anti-HA antibody was used for western blot. Total proteins were detected by Ponceau S staining. e-f, Phosphorylation of SWEET11 and SWEET12 under mannitol or ABA treatment in SWEET11pro::SWEET11-HA (e) and SWEET12pro::SWEET12-HA (f) transgenic plants. Total proteins were separated in a Phos-tag gel, and HA-tagged SWEET were detected with anti-HA antibody. g, Phosphorylation of SWEET11 and SWEET12 under ABA treatment in protoplasts of Col-0 and snrk2.2/3/6 (snrk2). Total proteins were separated in a Phos-tag gel, and FLAG-tagged SWEET were detected with an anti-FLAG antibody. Actin was used as a loading control. The experiments in (b-g) were repeated independently at least two times with similar results.
