Table 1 Effects of light conditions and mutagen application on the recovery of mitochondrial mutants

From: Targeted introduction of heritable point mutations into the plant mitochondrial genome

 

16 h light / 8 h dark

24 h light / 0 h dark

Treatment

None

EtBr

NEU

None

EtBr

NEU

1st reg. round

0/10

2a/10

0/10

0/10

3b/10

0/10

2nd reg. round

0/10

0/10

0/10

0/10

0/10

0/10

3rd reg. round

0/22

0/16

1c/19

0/24

1d/38

0/20

Total

0/42

2/36

1/36

0/44

4/58

0/40

  1. The numbers give the number of mutants obtained per number of regenerated shoots assayed. The concentration of EtBr was 0.001%, and that of NEU was 5 mM; reg. round, regeneration round.
  2. aLines S91G-2 and D93E/E94K-1.
  3. bInitially, two mutations were obtained. One gave rise to line Q89K-1; the other (T90N-1) was lost due to random mitochondrial genome segregation. However, one regenerated shoot later displayed a new mutation (line D93G-1) that was recovered.
  4. cLine V95I-1.
  5. dLine R85L-1.
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